Having gained chemical understanding at molecular level, chemistry graduates may choose to apply this knowledge in almost unlimited ways, as it can be used to analyze all matter and therefore our entire environment. 5326-23-8, Name is 6-Chloronicotinic acid, belongs to thiazines compound, is a common compound. In a pantent, author is Artym, Jolanta, once mentioned the new application about 5326-23-8, Category: thiazines.
Prolongation of skin graft survival in mice by an azaphenothiazine derivative
Azaphenothiazines are predominantly immunosuppressive compounds. We evaluated the efficacy of an azaphenothiazine derivative, 6-chloroetlaylureidoethyldiquino[3,2-b;2′,3′-e][1,4]thiazine (DQT) in prolongation of survival of skin allografts between BALB/c and C5781/6 mice. The mice were treated intraperitoneally (i.p.) with 100 mu g of DQT on alternate days, on days 1-13 of the experiment (7 doses). The effect of DQT on a two-way mixed lymphocyte reaction (MLR) in the human model, as well as its effect on production of TNF alpha and IL-10 in a whole blood cell culture, stimulated by lipopolysaccharide (LPS), were evaluated. In addition, DQT effects were investigated regarding the proportion of T cell subsets in human peripheral blood lymphocytes (PBMC) by flow cytometry. Lastly, the effect of DQT on expression of signaling molecules involved in pro apoptotic pathways was determined by RT PCR. The results showed that DQT significantly extended skin graft survival. The compound also strongly suppressed two-way MLR in the human model at a concentration range of 2.5-5.0 mu M. In addition, DQT inhibited LPS-inducible TNF alpha, but not IL-10 production. The compound preferentially caused a loss of the CD3-CD8 +CD11b + PBMC cell subset, and transformed CD3 + CD8 +(high) into CD3 + CD8+(low) cells. Lastly, we demonstrated significant increases in expression of caspases (in particular caspase 8) and of p53 in a culture of Jurkat T cells. We conclude that the immunosuppressive actions of the compound in allograft rejection may be predominantly associated with induction of cell apoptosis and inhibition of TNF a production. The apoptosis could be predominantly selective for the CD3-CD8 + CD11b + cell phenotype.
Do you like my blog? If you like, you can also browse other articles about this kind. Thanks for taking the time to read the blog about 5326-23-8, Category: thiazines.
Reference:
Thiazine – an overview | ScienceDirect Topics,
,Thiazine | C4H5NS – PubChem