Research speed reading in 2021.The potential utility of systematic synthetic strategy will be applicable to efficient generations of chemical libraries of compounds to find ‘hit’ molecules. 92-30-8, Name is 2-(Trifluoromethyl)-10H-phenothiazine, belongs to thiazines compound, is a common compound. In a pantent, author is Choukade, Ritumbhara, once mentioned the new application about 92-30-8, Recommanded Product: 2-(Trifluoromethyl)-10H-phenothiazine.
Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation
The present study describes green synthesis of silver nanoparticles (AgNPs) and inulin hydrolyzing enzyme nanocomplexes (ENC) usingAzadirachta indica(Ai) andPunica granatum(Pg) leaf extracts. Surface topology and physico-chemical characteristics of AgNPs were studied using surface plasmon resonance (SPR), FTIR, SEM, AFM and EDX analyses. Particle size analysis using dynamic light scattering and AFM studies revealed thatAi-AgNPs (76.4 nm) were spherical in shape having central bigger nano-regime with smaller surroundings whilePg-AgNPs (72.1 nm) and ENCs (Inulinase-Pg-AgNPs similar to 145 nm) were spherical particles having smooth surfaces.Pg-AgNPs exhibited significant photocatalysis of a thiazine dye, methylene blue. BothAi-andPg-AgNPs showed selective antibacterial action by inhibiting pathogenicBacillus cereus, while the probioticLactobacillusstrains remained unaffected.Ai-AgNPs showed potential anti-biofilm effect (30% viability) onB. cereusbiofilms.Pg-AgNPs showed anti-cancer effect against human colon cancer cell lines (Caco-2) resulting in 40% cell death in 48 h. Enzymes (inulinase, L-asparaginase and glucose oxidase) were successfully immobilized onto nanoparticles together with the biogenic synthesis of AgNPs and recyclability of the Inulinase-Pg-AgNPs complex was demonstrated. The study elaborates characteristics of green synthesized nanoparticles and their potential applications as anti-cancer, antibacterial and antioxidant nano drugs that could be used in food and nutraceutical industries. Enzyme immobilization on AgNPs without any toxic cross-linker opens up newer possibilites in enzyme-nanocomplex research.
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Reference:
Thiazine – an overview | ScienceDirect Topics,
,Thiazine | C4H5NS – PubChem